Effects of antimicrobials on Shiga toxin production in high-virulent Shiga toxin-producing Escherichia coli
نویسندگان
چکیده
Antimicrobial treatment of Shiga toxin-producing Escherichia coli (STEC) infections is controversial because antimicrobials may stimulate toxin (Stx) production, and thereby increase the risk developing haemolytic uremic syndrome (HUS). Previous in vitro studies have shown this mainly caused by STEC serotype O157:H7. The aim study was to investigate induction Stx transcription production different serotypes isolated from severely ill patients, following their exposure six classes antimicrobials. We investigated 12 high-virulent strains, all carrying stx2a gene, Liquid cultures strains were incubated with sub-inhibitory concentrations used reverse-transcription quantitative PCR measure relative expression Stx2a mRNA an enzyme-linked immunosorbent assay quantify production. In general antibiotics tested showed only minor effects on transcriptional levels Stx2a. Ciprofloxacin but two while gentamicin, meropenem azithromycin did not induce any examined. O104:H4 that greatest extent responded antimicrobial Gentamicin, result elevated recommend finding further search for candidates future STEC.
منابع مشابه
Shiga toxin-producing Escherichia coli
Shiga toxin-producing Escherichia coli (STEC) cause hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS) in humans. Outbreaks are linked to bovine food sources. STEC O157:H7 has been responsible for the most severe outbreaks worldwide. However, non-O157 serotypes have emerged as important enteric pathogens in several countries. The main virulence factor of STEC is the production of Shig...
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1. Rautio M, Lonnroth M, Saxen H, Nikku R, Väisanen ML, Finegold SM, et al. Characteristics of an unusual anaerobic pigmented gram-negative rod isolated from normal and infl amed appendices. Clin Infect Dis. 1997;25(Suppl 2):S107–10. 2. Rautio M, Saxen H, Siitonen A, Nikku R, Jousimies-Somer H. Bacteriology of histopathologically defi ned appendicitis in children. Pediatr Infect Dis J. 2000;19:...
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1. Rautio M, Lonnroth M, Saxen H, Nikku R, Väisanen ML, Finegold SM, et al. Characteristics of an unusual anaerobic pigmented gram-negative rod isolated from normal and infl amed appendices. Clin Infect Dis. 1997;25(Suppl 2):S107–10. 2. Rautio M, Saxen H, Siitonen A, Nikku R, Jousimies-Somer H. Bacteriology of histopathologically defi ned appendicitis in children. Pediatr Infect Dis J. 2000;19:...
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ژورنال
عنوان ژورنال: Microbial Pathogenesis
سال: 2021
ISSN: ['0882-4010', '1096-1208']
DOI: https://doi.org/10.1016/j.micpath.2020.104636