Effects of antimicrobials on Shiga toxin production in high-virulent Shiga toxin-producing Escherichia coli

نویسندگان

چکیده

Antimicrobial treatment of Shiga toxin-producing Escherichia coli (STEC) infections is controversial because antimicrobials may stimulate toxin (Stx) production, and thereby increase the risk developing haemolytic uremic syndrome (HUS). Previous in vitro studies have shown this mainly caused by STEC serotype O157:H7. The aim study was to investigate induction Stx transcription production different serotypes isolated from severely ill patients, following their exposure six classes antimicrobials. We investigated 12 high-virulent strains, all carrying stx2a gene, Liquid cultures strains were incubated with sub-inhibitory concentrations used reverse-transcription quantitative PCR measure relative expression Stx2a mRNA an enzyme-linked immunosorbent assay quantify production. In general antibiotics tested showed only minor effects on transcriptional levels Stx2a. Ciprofloxacin but two while gentamicin, meropenem azithromycin did not induce any examined. O104:H4 that greatest extent responded antimicrobial Gentamicin, result elevated recommend finding further search for candidates future STEC.

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ژورنال

عنوان ژورنال: Microbial Pathogenesis

سال: 2021

ISSN: ['0882-4010', '1096-1208']

DOI: https://doi.org/10.1016/j.micpath.2020.104636